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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

A new CreERT2 line for studying PDGFRα-expressing cells in vivo


Title201609

A new CreERT2 line for studying
PDGFRα-expressing cells in vivo

B6.Cg-Pdgfra<tm1.1(EGFP/cre/ERT2)Hyma> (RBRC09616)

201605

Courtesy of Takumi Era, M.D., Ph.D.

E14.5 (top) and E20.5 (bottom) embryo of PDGFRα-GFP/CreERT2 mice crossed with ROSA26-Tomato (left) or ROSA26-LacZ (right) reporter mice. Cre activity was induced by intraperitoneal injection of 4-hydroxytamoxifen in pregnant female mice at E9.5.

 

Mesoderm and its descendant cells are required for organogenesis, wound repair, and disease pathogenesis. It can be classified into cells positive for vascular endothelial growth factor receptor 2 (VEGFR2 or FLK1) or platelet-derived growth factor receptor α (PDGFRα), representing lateral and paraxial mesoderm, respectively. Era and colleagues generated knock-in mice carrying GFP-Cre-ERT2 at the start codon of Pdgfra [1]. They confirmed Cre activity in mesoderm cells at gastrulation and the mesenchyme of E9.5. Neural crest cells expressing PDGFRα was also confirmed to contribute to the facial mesenchyme. PDGFRα-GFP/CreERT2 mouse strain is a useful tool to investigate novel role of PDGFRα-expressing cells and their descendant cells in vivo.

 

Depositor : Takumi Era, M.D., Ph.D.
Department of Cell Modulation
Institute of Molecular Embryology and Genetics, Kumamoto University
Strain name : B6.Cg-Pdgfra<tm1.1(EGFP/cre/ERT2)Hyma>
RBRC No. : RBRC09616
Reference : [1] Miwa H, Era T. Generation and characterization of PDGFRα-GFPCreERT2 knock-In mouse line. Genesis; 53(5):329-36, 2015.

 

September 2016
Contact: Shinya Ayabe, Ph.D.
Experimental Animal Division, RIKEN BioResource Center
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