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Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Monitoring cell replacement and migration in vivo with Kaede


Title201606

Monitoring cell replacement and migration
in vivo with Kaede

B6.Cg-c/c Tg(CAG-tdKaede)15Utr (RBRC05738)
B6.Cg-Tg(CAG-tdKaede)15Utr (RBRC05737)

201608

Courtesy of Yoshihiro Miwa, Ph.D.

Irradiation with 405 nm violet light to the lower body of Kaede mouse changed fluorescence from green to red.

 

Kaede is a photoconvertible fluorescent protein which was cloned from a stony coral Trachyphyllia geoffroyi. Kaede changes its color from green to red within a few minutes after exposure to violet light [1]. In transgenic mice carrying Kaede cDNA under the CAG promoter, all the cell types constitutively exhibit green fluorescence signal [2]. Immune cells in lymphoid organs and peripheral tissues in Kaede mice can be labeled in red by brief exposure to violet light [3]. Kaede mice are a useful tool to monitor cell replacement at the photoconverted site and/or cell migration to other regions in the body.

 

Depositor : Yoshihiro Miwa, Ph.D.
Faculty of Medicine
University of Tsukuba
Strain name : B6.Cg-c/c Tg(CAG-tdKaede)15Utr
RBRC No. : RBRC05738
Strain name : B6.Cg-Tg(CAG-tdKaede)15Utr
RBRC No. : RBRC05737
References : [1] Ando R, Hama H, Yamamoto-Hino M, Mizuno H, Miyawaki A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc Natl Acad Sci USA; 99(20):12651-6, 2002.
[2] Tomura M, Yoshida N, Tanaka J, Karasawa S, Miwa Y, Miyawaki A, Kanagawa O. Monitoring cellular movement in vivo with photoconvertible fluorescence protein “Kaede” transgenic mice. Proc Natl Acad Sci USA; 105(31):10871-6, 2008.
[3] Tomura M, Honda T, Tanizaki H, Otsuka A, Egawa G, Tokura Y, Waldmann H, Hori S, Cyster JG, Watanabe T, Miyachi Y, Kanagawa O, Kabashima K. Activated regulatory T cells are the major T cell type emigrating from the skin during a cutaneous immune response in mice. J Clin Invest.; 120(3):883-93, 2010.

 

August 2016
Contact: Shinya Ayabe, Ph.D.
Experimental Animal Division, RIKEN BioResource Center
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