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Whole-body in vivo monitoring using B6.Cg-Tg(IL6-luc)Mym Tyr<c-Brd> (RBRC09487)
Courtesy of Masayuki Yamamoto, M.D., Ph.D. Robust Luc-luminescence in brain and spinal cord of WIM-6 mouse subjected to experimental autoimmune encephalomyelitis (EAE); in vivo imaging analysis from dorsal view and 3D-computed tomography. |
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IL-6 is a pleiotropic cytokine that is produced in many types of cells upon inflammatory insult [1]. IL-6 functions as a warning signal in the event of infection and tissue damage, therefore has been considered to be a reliable inflammatory marker. Yamamoto and colleagues generated bacterial artificial chromosome (BAC)-based hIL6-luciferase transgenic mice to evaluate inflammatory status in vivo (i.e., WIM-6 mouse; Whole-body in vivo monitoring using human IL6-luciferase transgenic mice) [2]. In the LPS-induced sepsis model, LPS dose- and time-dependent luciferase induction was detected by whole-body monitoring and in vitro luciferase assay using bone marrow-derived macrophages. In experimental autoimmune encephalomyelitis (EAE), WIM-6 mice exhibited robust induction of luciferase luminescence in brain and spinal cord. WIM-6 system also monitored therapeutic efficacy of anti-inflammatory reagents in atopic dermatitis model. Overall, our results demonstrate that WIM-6 mouse system serves as a useful tool for in vivo monitoring of inflammation in various disease models. |
| Depositor | : | Masayuki Yamamoto, M.D., Ph.D. Department of Medical Biochemistry Tohoku University Graduate School of Medicine |
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| Strain name | : | B6.Cg-Tg(IL6-luc)Mym Tyr<c-Brd> | |
| RBRC No. | : | RBRC09487 | |
| References | : | [1] | Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease.Cold Spring Harb Perspect Biol.,; 6(10):a016295, 2014. |
| [2] | Hayashi M, Takai J, Yu L, Motohashi H, Moriguchi T, Yamamoto M. Whole-Body In Vivo Monitoring of Inflammatory Diseases Exploiting Human Interleukin 6-Luciferase Transgenic Mice.Mol Cell Biol.; 35(20):3590-601, 2015. | ||
| May 2016 Contact: Shinya Ayabe, Ph.D. Experimental Animal Division, RIKEN BioResource Center All materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content. |
