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November 2007 Mouse of the Month |
EGFP-Akt Transgenic Mouse
A model for visualization of PI(3,4,5)P3 dynamics
BRC No. 01155_EGFP-Akt-2 transgenic mouse
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| Research Application | : | Cancer Research, Cell Biology Research, General Purpose, Metabolism Research, and Immunology and Inflammation Research |
 BRC No.01156 EGFP-Akt-3 transgenic mouse |
 Concentrated fluorescent activity of AktPH-GFP at the leading edge (Arrow Heads) during chemotactic peptide-stimulated neutrophil migration was visualized by confocal fluorescence microscopy. |
PI3Ks (phosphoinositide 3-kinases) regulate many critical cellular responses by producing PI(3,4,5)P3 (phosphatidylinositol 3,4,5-trisphosphate). The EGFP-Akt transgenic mice express a fluorescent bioprobe for PI(3,4,5)P3/PI(3,4)P2 (phosphatidylinositol 3,4-bisphosphate) which is the pleckstrin homology domain of Akt (AktPH) and EGFP fusion protein. The AktPH-GFP fluorescence shows defused localization in cytoplasm, however the fluorescence is concentrated at the leading edge during fMet-Leu-Phe (N-formylmethionyl-leucylphenylalanine; chemotactic peptide)-stimulated neutrophil migration. These transgenic mice are useful for studying PI(3,4,5)P3 metabolism in physiological and pathological situations by crossing your mutant mice.
| Depositor | : | Dr. Takehiko Sasaki (Akita University) (Japanese page) |
| References | : | Nature Cell Biol. (2007) 9(1):36-44. Biochemical Society Transactions (2007) 35(Pt 2):215-8. |
