BRC facility Health Report
Update information
キーワード:
※1. しぼり込み検索をする場合は、スペース(半角空白文字)で区切って単語を入力して下さい。
  
IMPC 遺伝子・表現型 検索

よく使われるリソース

サービス

品質管理

技術情報

カスタマーサービス

ビデオプロトコル

Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
JoVE 3155 11/18/2011

Dec 2007 Nanog-GFP Transgenic Mouse


mouse_of_month_200712

Nanog-GFP Transgenic Mouse
A tool for generation of germline-competent iPS cells

BRC No. 02290_STOCK Tg(Nanog-GFP,Puro)1Yam
Research Application : Stem Cell Research
Developmental Biology
mn0712_0101
Generation of iPS cells by using Nanog-GFP Tg mouse (Nature, 448, 313-317 (2007))

 

mn0712_0103 mn0712_0102
Germline-competent iPS cells from Nanog-GFP transgenic mouse.
Phase contrast (left) and fluorescence (right) micrographs are shown.
  Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror) cassette into the 5′ untranslated region of a bacterial artificial chromosome (BAC) containing the mouse Nanog gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency.
The iPS cells will be available soon from RIKEN BRC Cell Bank.

 

Depositor : Dr. Shinya Yamanaka (Kyoto University)
Reference :  Nature (2007) 448:313-7.