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February 2007 Mouse of the Month |
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FLPe transgenic mouse A tool for conditional genetic manipulation BRC No. 01834_B6-Tg(CAG-FLPe)36
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 BRC No. 01834_B6-Tg(CAG-FLPe)36 |
 FLP-FRT system: Floxed mice can be generated after excision of neo (selective marker gene) by crossing with FLP Tg mice. |
The FLP-FRT system is similar to the cre-loxP system and is useful for more complex dual approaches that combine FLP with other recombinases, such as Cre and ΦC31.. FLPe is a variant of the FLP recombinase with a higher enzymatic activity at 37°C. This transgenic mouse of C57BL/6 strain expresses the FLPe recombinase under the control of the CAG promoter. This strain can be used as ‘FLP deleter’ mouse by crossing with the other genetically-engineered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest.
| Depositor | : | Shigeyoshi Itohara (RIKEN BSI) |
| Reference | : | Kanki H, Suzuki H, Itohara S. High-efficiency CAG-FLPe deleter mice in C57BL/6J background. Exp. Anim., 55, 137-141 (2006). |
