June 2015 Genetic tool for controlled G-CaMP7 expression in vivo


Genetic tool for controlled G-CaMP7 expression in vivo

C57BL/6J-Tg(tetO-G-CaMP7,-DsRed2)572Bsi (RBRC06510)


Courtesy of Masaaki Sato, Ph.D. and Yasunori Hayashi, M.D., Ph.D.

Expression patterns of G-CaMP7 (top, green), coexpressed DsRed2 (middle, red), and G-CaMP7 overlaid with DsRed2 and Hoechst nuclear counterstaining (bottom, blue) in adult TRE-G-CaMP7 × CaMKII-tTA mice. Parasagittal sections cut at different mediolateral levels are shown from left to right.


Ca2+ is an intracellular messenger that regulates cellular functions such as excitability, gene expression, secretion, and contraction. Genetically encoded calcium indicators (GECIs), such as GFP-based G-CaMPs, produce great changes in fluorescence in response to increases in intracellular Ca2+ concentrations. Understanding brain function requires techniques for monitoring the patterns of activity in neuronal networks, and many transgenic mouse lines that express G-CaMPs in the brain have been reported. Hayashi and colleagues generated a new transgenic strain that expresses the improved G-CaMP variant G-CaMP7 [1] under a tetracycline response element (TRE) [2]. Crossing with tTA driver mice and doxycycline treatment results in cell type-specific and temporally controlled expression of G-CaMP7 in TRE-G-CaMP7 mice. These technical advances will provide a powerful approach for functional and pharmacological studies of neural circuit activity in vivo..


Depositors : Masaaki Sato, Ph.D.
Japan Science and Technology Agency (JST)
Yasunori Hayashi, M.D., Ph.D.
RIKEN Brain Science Institute (BSI)
Strain name : C57BL/6J-Tg(tetO-G-CaMP7,-DsRed2)572Bsi
RBRC No. : RBRC06510
References : [1] Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J. Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLOS ONE; 7(12):e51286, 2012.
[2] Sato M, Kawano M, Ohkura M, Gengyo-Ando K, Nakai J, Hayashi Y. Generation and imaging of transgenic mice that express G-CaMP7 under a tetracycline response element. PLOS ONE; 10(5):e0125354, 2015.


June 2015
Contact: Shinya Ayabe, Ph.D.
Experimental Animal Division, RIKEN BioResource Center
All materials contained on this site may not be reproduced, distributed, displayed, published or broadcast without the prior permission of the owner of that content.